Establishment and evaluation of the sdf-1α over-expressing mouse parkinson disease model

In the present study, to establish a stromal cell derived factor-1α (SDF-1α) mouse Parkinson’s disease (PD) model, low-dose 1-methyl-4-phenyl-tet-rahy dropyridine (MPTP) was subcutaneously injected at different time points into mice which had been intracerebrally treated with pDsRed2-N1-SDF-1α, and behaviors were observed at different time points. The pod-grabbing time, the number of tyrosine hydroxylase (TH) positive cells and the expression of SDF-1α in the brain were determined at different time points. PD-like behaviors were observed in the mice of MPTP group and could last for at least 40 days. At different time points, the pod-grabbing time in MPTP group was longer than that in control group, but the TH positive cells and DA content were markedly decreased when compared with control group. In addition, plasmid transcription was noted in MPTP group and SDF-1α expression was detectable. Intermittent and multiple subcutaneous injections of MPTP can be used to establish a PD model in SDF-1α over-expressing mice, which provides an experimental basis for the investigation of the role of SDF-1α induced chemotaxis and migration in the treatment of PD.